广州健仑生物科技有限公司

广州健仑生物科技有限公司

广州健仑长期供应：动物、畜牧、食品、药品、化妆品、水产品、违禁品的快速检测试剂盒。

动物类的主要检测项目包括：猪、狗、猫、牛、鸡、鸭和鹅的传染病。

畜牧类的主要检测项目包括：多种添加剂、瘦肉精添加剂等。

食品类的主要检测项目包括：添加剂残留、农药残留、药物残留等。

化妆品的主要检测项目包括：重金属、有害物质等。

水产品的主要检测项目包括：呋喃类药物残留、抗生素残留等。

违禁品的主要检测项目包括：MOP/MET/KET/THC/MDMA/COC/BZO/AMP/K2/BAR/TCA/BUP/MTD/PCP/OXY/EDDP

我司还提供其它进口或国产试剂盒：包括传染病系列、免疫组化系列、诊断血清等产品欢迎致电。

欢迎电话咨询13802525278

欢迎QQ咨询2042552662

黄曲霉素B1检测卡（饲料霉菌检测试剂）

黄曲霉素B1检测卡（饲料霉菌检测试剂）

想了解更多的产品及服务请扫描下方二维码：

【公司名称】 广州健仑生物科技有限公司

【市 场 部】 13802525278 020-82574011  杨永汉

【公司传真】 020-32206070

【腾讯Q Q】 2042552662

【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

3 ultrasonic treatment: a certain amount of power ultrasound treatment of cell suspension, the sharp shock cells rupture, this method is more suitable for microbial materials, the preparation of various enzymes using E. coli, often selected with 50-100 milliliters of bacteria / ml concentration, In the 10KG to 10KG frequency processing 10-15 minutes, the disadvantage of this method is the treatment process will produce a lot of heat, cooling measures should be taken accordingly, the time and the ultrasonic intermittent time, ultrasonic time can be adjusted, the ultrasound completely bacterial liquid should Become clear, if you can not rest assured under the microscope. Ultrasonic and heat-sensitive proteins and nucleic acids should be used with caution.

4. repeated freeze-thaw method: the cells frozen at -20 degrees below room temperature melt, repeated several times, due to intracellular ice formation and residual cell liquid salt concentration caused by swelling, the cell structure broken.

5. Chemical treatment: Some animal cells, such as tumor cells can be sodium dodecyl sulfate (SDS), sodium deoxycholate and other cell membrane damage, bacterial cell wall thick, lysozyme enzyme treatment can be better, I The concentration used is generally 1 mg / ml.

No matter which method is used to break up the tissue cells, the intracellular proteins or nucleic acid hydrolases are released into the solution to degrade the macromolecules, resulting in the reduction of the amount of natural products. The addition of diisopropylfluorophosphate (DFP) can inhibit Slow down the autolysis; add iodoacetic acid can inhibit those activity centers need to have a sparse base proteolytic enzyme activity, join the benzene type influenza (PMSF) also clears the proteolytic enzyme activity, but not all, and should be broken At the same time several times; In addition, you can also choose pH, temperature or ionic strength, so that these conditions must be suitable for the extraction of the target substance.