广州健仑生物科技有限公司

广州健仑生物科技有限公司

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赭曲霉素A检测卡（饲料霉菌检测试剂）

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If the lysozyme effect is okay, I think it may be better to use a lysozyme to initially lyse the cells and then break the macromolecular nucleic acids further with ultrasound to reduce the viscosity. Ultrasound may denature the protein, but lysozyme alone can not determine whether it is completely broken, but also add nuclease to reduce viscosity. This two-step strategy should also reduce the time needed to optimize the crushing conditions.

I use the lysozyme placed for a long time, it may be deactivated. I have just set a new life, but I have to buy the day after tomorrow, I hope it is useful.

How to observe whether lysozyme has been lysis of cells, by observing whether the change in viscosity?

only repeated freezing and thawing can effectively lyse E. coli cells?

Lysozyme only in the pH value of greater than 8.0 under the conditions of play a bacteriolytic effect, be sure to keep the PBS pH> 8.0, while the amount of lysozyme must be sufficient!

After the addition of lysozyme, it is best placed in a magnetic stirrer for 30 minutes, followed by sonication, my sonication conditions: power 400W, work 2 seconds, 2 seconds interval, repeat 199 times. Cells were resuspended in 500 ml of culture and resuspended in 30 ml of PBS. Ultrasound efficiency was still acceptable.