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玉米赤霉烯酮检测卡（饲料霉菌检测试剂）

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I use bio-lysozyme, said dry powder 20mg. 200ml of bacteria solution was resuspended with 18 ml of NaCl, added with 2 ml of 25mM Tris-HCl (pH8.0), the lysozyme powder was added to the system, mixed, the pH was reduced to 5-6, plus 20 ul 2M Tris base, pH Rise to ~ 8.4C for 1 hour, the supernatant liquid became clear, shake the system did not become sticky. Measured pH is still around 8. 37C and then incubated for 1 hour, has not changed, I simply do not know what to do.

Another 200 ml culture was treated with lysozyme for 1 hour and then sonicated. Conditions: 300W, 5 seconds over 5 seconds to stop, repeat 99 times. Bacterial fluid changes, but not obvious. Continue to 400 ultrasound, from the appearance point of view is not much difference. I just want to say tmd. Hell

What is wrong with my operation, please correct me.

Is the manufacturer's requirement for lysozyme important?

My inducer was from a 1:20 overnight culture inoculation, 3 hours after 37C growth for 2 hours at 30 C (0.8 mM IPTG).

Is the bacterium too strong, Pharmacia's instructions 200 ml culture was resuspended with 10 ml, and I used 20 ml, still not enough lean? Someone told me that mycotic fluid should be diluted, 200 ml with 30-40 ml resuspended. But the actual operation is not ideal.

SDS-PAGE always observed the effect of cell disruption is not complete enough. Ultrasound so many times, the protein should be ultra-fine degeneration.