广州健仑生物科技有限公司

广州健仑生物科技有限公司

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动物类的主要检测项目包括：猪、狗、猫、牛、鸡、鸭和鹅的传染病。

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呕吐毒素检测卡（饲料霉菌检测试剂）

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4, ultrasonic conditions of choice

Ultrasonic crushing conditions can not be generalized, depending on your experimental requirements, some are broken bacteria, some are broken cells, to master the power and each ultrasound time, power, each ultrasound time can be shortened, can not let the temperature rise High, if necessary, sonicated under ice bath conditions. It is not a question of whether or not foaming occurs with each ultrasound, which is clearly related to the amount of your ultrasound.

5, how to identify the degree of bacterial ultrasonic disruption

The easiest way: smear - Gram crystal violet solution staining 0.5 minutes --- microscopic observation

Remember: only crystal violet staining, do not forget to wash slightly rinse with water after staining

6, DNaseI bacterial lysis

Different purity of the enzyme conversion standards, so you better check which company's enzyme? Look carefully at the instructions, there may be conversion instructions.

Also look at the reference enzyme used which company, to the company's home page may also be reaped.

I have used gorgeous and TAKARA DNase, beautiful is in mg / ml. China and the United States also have RNase free DNase, quantitative activity units. Both enzymes of TAKARA are quantified in active units.

When I lyse bacteria by sonication, I add some DNase, generally using a pre-experiment with different amounts of enzyme plus ultrasound and selecting the amount of enzyme that meets your needs.

In fact, depending on the purpose and method, the required amount of enzyme is also adjustable, such as enzyme digestion temperature (16 degrees or 37 degrees).