广州健仑生物科技有限公司

广州健仑生物科技有限公司

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伏马菌素B1检测卡（饲料霉菌检测试剂）

伏马菌素B1检测卡（饲料霉菌检测试剂）

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7, ultrasonic cracking bacteria is complete?

The easiest way: smear - Gram crystal violet solution staining 0.5 minutes --- microscopic observation

Remember: only crystal violet staining

Second, inclusion body washing

1, inclusion body washing problems

The usual washing method is generally not clean, I used to do so, the first inclusion body with 6M guanidine hydrochloride fully dissolved, filtered to remove undissolved material, pay attention to run sample run electrophoresis, and then diluted with water to 4M, centrifuged Precipitation and supernatant were run electrophoresis, and so on can be diluted to the right concentration, you can find a suitable way to remove impurities, in fact, this is the gradient precipitation method, I think than the usual direct elution effect.

Inclusion body generally difficult to dissolve, so you have to pay attention to the undissolved part, you can run electrophoresis contrast, because sometimes it is difficult to dissolve your target protein, so each treatment should run the supernatant and sediment run electrophoresis, so that the target Protein lost.

In addition, the newly treated inclusion body is well dissolved. Difficult to dissolve after freezing, dissolution also takes a long time, but also requires a lot of solvent. If it is a lot of insoluble is not what you want, then do not control it.

2, how to get more pure inclusion body

For inclusion body purification, inclusion body pre-treatment is very important.